Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.074844 |
Chromosome: | chromosome 17 |
Location: | 2085579 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g711401 | (1 of 10) IPR006593 - Cytochrome b561/ferric reductase transmembrane | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGCCAGGGGGGGGGTAGTTTCGACTGGGAAAGGCCCCATGTTCTAA |
Internal bar code: | ACACGGTCACGCGCGTCTTCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 873 |
LEAP-Seq percent confirming: | 29.6296 |
LEAP-Seq n confirming: | 8 |
LEAP-Seq n nonconfirming: | 19 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTGCCGGAAGAGATTGGTC |
Suggested primer 2: | TTGGAAGGGGTCATCATGCC |