Insertion junction: CLIP2.074850_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.074850
Chromosome:chromosome 6
Location:3711326
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278157 (1 of 1) 3.2.1.143 - Poly(ADP-ribose) glycohydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACTCGTGCACCTGCCCACCTTTGCTCGCTGTCTGAGCGGTCGGACAC
Internal bar code:ACTGCCTCTACTCTTCTTTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1422
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCTGTGCTATTCTGCGC
Suggested primer 2:CTTCTCACCAGCTCCACCTG