| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.074854 |
| Chromosome: | chromosome 10 |
| Location: | 5905105 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACCCTGCTTTGCCCCTGCTGCTGGACTCCCGGCCCCCCGTCATGC |
| Internal bar code: | AGTAAAGTCAGGTAGAATGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2299 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGTGAGTACCGTGCAGT |
| Suggested primer 2: | TCACATTCATGGCACCGACA |