| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074860 |
| Chromosome: | chromosome 6 |
| Location: | 5004435 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTTAGTGTTAGGTACCATTATCCCGAGCATGTAAGAATACCACATGC |
| Internal bar code: | GCAAGACACTGAGGCGCGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2969 |
| LEAP-Seq percent confirming: | 29.7297 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATAGCACACACACACCCC |
| Suggested primer 2: | TCCCAGACCCTTGCGAGATA |