Insertion junction: CLIP2.074865_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074865
Chromosome:chromosome 6
Location:3014238
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g274450 SMM20 (1 of 5) PTHR13271//PTHR13271:SF28 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED; S-adenosyl-L-methionine-dependent methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGTGAAGGCGCTGCCTTTATGTGCACAGTCCACACACTCCAGTCGTG
Internal bar code:TCCAGGTTAAAACGACTCTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1968
LEAP-Seq percent confirming:92.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCCAACCTCCTCTCCATA
Suggested primer 2:CATATCACTGGGTCGCCCTC