| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074866 |
| Chromosome: | plastome |
| Location: | 193600 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802334 | orf528 | unknown ORF | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTCGAAGCGGAAAGTATGTCTCCCCTTTGGGATATTTATGTACTTC |
| Internal bar code: | GTTTTAATCTCATCGCCTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 25.4902 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCTTTGGGGTTGCACAG |
| Suggested primer 2: | CCAGTAGCTTTAGGTGCAAACC |