| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074870 |
| Chromosome: | chromosome 12 |
| Location: | 5557712 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCATGGATATCCGTGCACGCGTGGAATGTAAAGGAGAACCGCGGTCTG |
| Internal bar code: | GAAAGTCCAAGACGTGGATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3104 |
| LEAP-Seq percent confirming: | 84.2857 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTGCTTGCTTGCGTTGA |
| Suggested primer 2: | CAAGCAGGTTGTTGAGTGCC |