| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074872 |
| Chromosome: | chromosome 2 |
| Location: | 4961136 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110350 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | 3'UTR | |
| Cre02.g110400 | (1 of 1) PTHR12732:SF0 - PCI DOMAIN-CONTAINING PROTEIN 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTATCCGACAACTGTGAGATGCGACACGACGTGTGCGACGGTCGGTTG |
| Internal bar code: | AGAGGGGGAGCTTCCATATAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4304 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTAAACCGCCTTCTCA |
| Suggested primer 2: | CCCTACGTACCGCTAAGCAG |