Insertion junction: CLIP2.074874_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.074874
Chromosome:chromosome 13
Location:949316
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g568100 (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGAACTGACGGCGAGACGCGCTCGAGGACAAGCTGGCGACCCACGGG
Internal bar code:GGAAAGTAGAGAGATGATGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:821
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAAGCATGGTCCAGAAGT
Suggested primer 2:GTCCGCACTTGTACTGTCGG