Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.074874 |
Chromosome: | chromosome 13 |
Location: | 949324 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g568100 | (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCACCCGCTGCCATGCGCGACGGCAGCGTTGGGCTGCCGCGGCCCGGG |
Internal bar code: | GGAAAGTAGAGAGATGATGGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1462 |
LEAP-Seq percent confirming: | 32.5581 |
LEAP-Seq n confirming: | 14 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCCGCACTTGTACTGTCGG |
Suggested primer 2: | CCCAAGCATGGTCCAGAAGT |