Insertion junction: CLIP2.074883_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074883
Chromosome:chromosome 6
Location:5372398
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g285300 SUT1 Sugar transporter; (1 of 1) K08200 - MFS transporter, OCT family, solute carrier family 22 (organic cation transporter), member 3 (SLC22A3, OCT3) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCGCAAAACATGTAGTCTCGAACCACGTCCGCAAGCAGAACAGCTTG
Internal bar code:AAGGGAGGATTGTGTATCTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:6204
LEAP-Seq percent confirming:69.5652
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCTCGGGATTTGGAACTG
Suggested primer 2:AGCCAAATACGGGAAGGGTG