| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074895 |
| Chromosome: | chromosome 3 |
| Location: | 5229783 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181950 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAAACGCCATGCGTCTCAACGCATAGGGCGTAGGCGGTCCCGTCACT |
| Internal bar code: | GAGACTCTACTGTCGACGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4326 |
| LEAP-Seq percent confirming: | 81.3333 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGGGTGCATGACAACAC |
| Suggested primer 2: | ACATGTGATGCCCCTTCTGG |