| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074901 |
| Chromosome: | chromosome 6 |
| Location: | 8579465 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309717 | (1 of 1) PTHR12735//PTHR12735:SF22//PTHR12735:SF3 - BOLA-LIKE PROTEIN-RELATED // SUBFAMILY NOT NAMED // PROTEIN T12D8.10-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGGGCCGCAAACACTGCAACCACCATTGCGAACAGGCCTTGCAAGCT |
| Internal bar code: | CTATATTTGCGTTCTCTTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3800 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACGCACTTCAAGGTGGA |
| Suggested primer 2: | AGAGTGTGTGCGGCTTTGTA |