Insertion junction: CLIP2.074907_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.074907
Chromosome:chromosome 7
Location:4509250
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g343650 (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC 3'UTR
Cre07.g343700 OGD2 Dihydrolipoamide succinyltransferase, oxoglutarate dehydrogenase E2 component; (1 of 2) K00658 - 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase) (DLST, sucB) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGACACAAGGCGGTTTGGGGGCTTCTCCATAGGAATTTGGACAAAACC
Internal bar code:TCGTCTACAGATATATCATAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1488
LEAP-Seq percent confirming:97.1429
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTTCCTTGTCGTGTCAGG
Suggested primer 2:GCATGACGCGGGAATAGGTA