Insertion junction: CLIP2.074929_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.074929
Chromosome:chromosome 16
Location:7375302
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g679893 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED 3'UTR
Cre16.g680005 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCCATGCACATCCCCCTTACGTCGCTACTCATAACAAGTTTATGCATA
Internal bar code:ACAACTTGGCGTCGAAAGCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3412
LEAP-Seq percent confirming:99.0385
LEAP-Seq n confirming:103
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGGGTAGAAGTCCAGGC
Suggested primer 2:GCGGTGAAAAGGCACACAAT