| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074955 |
| Chromosome: | chromosome 1 |
| Location: | 253441 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001650 | Predicted ankryin-repeat containing protein; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGCAGTGGGTCAGTGGGGTTGTGACAGCCCTCAGCCCAGGCAGCCCC |
| Internal bar code: | TCCACTGATTCAATGATTCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 3.7037 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCAAAGCGGAGGAAGGGA |
| Suggested primer 2: | AGCAGTACAGTGGCACATCC |