| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074970 |
| Chromosome: | chromosome 10 |
| Location: | 588948 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801104 | (1 of 1) IPR000104//IPR003072//IPR004333 - Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type // Transcription factor, SBP-box | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTGCTGCTGCCGCCGCTGCTGCTACTGCTGCTCTGTCCGACCTTGGC |
| Internal bar code: | ACTTACACCATCTGGAGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATACAACATCGGCGTTC |
| Suggested primer 2: | ACTACTGCAGCACCATGTCC |