| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.074994 |
| Chromosome: | chromosome 12 |
| Location: | 2010082 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801373 | (1 of 2) IPR000104//IPR000477//IPR005135 - Antifreeze protein, type I // Reverse transcriptase domain // Endonuclease/exonuclease/phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGAGGGCTCCCGGTCTGGTGAAGCGTGTGCGGATATGTATGTGACT |
| Internal bar code: | GTGTCCTGGGCTTTAGTAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5643 |
| LEAP-Seq percent confirming: | 41.9355 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTGCAGTCCCAAAACCA |
| Suggested primer 2: | GTTTGATGCCAGCCCACATG |