| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075000 |
| Chromosome: | chromosome 13 |
| Location: | 4593789 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCAAACCTCCAGGAGTTTGCCGGACCAGTTTTGAAAGTCTGTGCGCG |
| Internal bar code: | TATACCCTCCCGTTTTCAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2677 |
| LEAP-Seq percent confirming: | 74.1936 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGGTTTGGTTGCTTCTG |
| Suggested primer 2: | GTTGTGTCGGTTGTGTCACG |