| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075002 |
| Chromosome: | chromosome 9 |
| Location: | 6001202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412450 | RAD21 | (1 of 3) PF04824 - Conserved region of Rad21 / Rec8 like protein (Rad21_Rec8); cohesin complex subunit, putative mitotic isoform | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGTGAGATGCTGCCCGCTCCGTGTGCTCAGAGCAACAGGCAGCCACA |
| Internal bar code: | CTACGTGGCATTAAGAGACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3838 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAACAAAACCCGTGGTG |
| Suggested primer 2: | GCCCCAATAGAGCTGGTGTT |