| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075008 |
| Chromosome: | chromosome 13 |
| Location: | 3505070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587600 | (1 of 2) PTHR10907//PTHR10907:SF33 - REGUCALCIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTAACTCCTCAGCGGCTCTCAGATTCACGGCACCCTCGCTGGGCCCTA |
| Internal bar code: | GTATATTGTGCTGGCCTGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1243 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGTGAGTCGATGGAGCG |
| Suggested primer 2: | TGTGCTGAGGAGACGAACAC |