| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075040 |
| Chromosome: | chromosome 6 |
| Location: | 841140 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255600 | EZY24,EZY1,EZY1A | Copy A in the EZY1 tandem gene cluster | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCTTTATCGTACGTTGCCATGCCCATACTTGGGGCGCGGGGGCGGCC |
| Internal bar code: | AACTATCACAGTAAAGGAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4488 |
| LEAP-Seq percent confirming: | 16.4706 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGAGTGAGTGAGTGCAG |
| Suggested primer 2: | GCTATGTGTGCTGAACCCCT |