| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075045 |
| Chromosome: | chromosome 12 |
| Location: | 8881348 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546100 | FAP125,KIN14-2 | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Flagellar Associated Protein 125 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACAATAGAAGGGGAAACAAGCCTAGCCCATTGTCTAAAACTCTAAGGG |
| Internal bar code: | GAATTGGGATGTAAAAGGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2315 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATCCGTTGACGAGGGTAG |
| Suggested primer 2: | AGATCCTGAGTGCTCCGTCT |