| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075050 |
| Chromosome: | chromosome 3 |
| Location: | 3201436 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165200 | APG7 | Autophagy-related protein, ubiquitin-activating enzyme E1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCTCAAGCACATACAGCTTGCCGCCCGCCTACCCGCCCGGCCACCGG |
| Internal bar code: | TAGGCGTTATTTTAATGAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 65.2174 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCTTCCTCCAACTCA |
| Suggested primer 2: | AAGTGATGTCTCGGCACAGG |