Insertion junction: CLIP2.075060_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.075060
Chromosome:chromosome 3
Location:9174021
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCTCCAGCATAACGTATACGCCAACGCCGTTTGCTGAGTCCGTGGTT
Internal bar code:AGAGGGAATTGTCCGAAGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:5092
LEAP-Seq percent confirming:88.5246
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGTTGGCAACGAGTGTG
Suggested primer 2:TACTTGTGTCTGACCACCGC