| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075064 |
| Chromosome: | chromosome 6 |
| Location: | 2415619 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268700 | POB28 | Proteome of basal body 28; (1 of 1) PTHR18937//PTHR18937:SF224 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCGATGGCCGCATGTTGCACCAAGCGGAAGCTTGCGATCGGCTGTGG |
| Internal bar code: | GTCATTAGATGGTTGTTGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1068 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCGTTTGGGAGGAGCAC |
| Suggested primer 2: | TTGACATTGCTGCACACGTG |