| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.075067 |
| Chromosome: | chromosome 5 |
| Location: | 3116027 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755797 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAAGCTTGCGGTTGTTTGGACCGTATGGGCCCGCGGCTATGCGGCAG |
| Internal bar code: | CCTCAATATTGGTGCCATAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2650 |
| LEAP-Seq percent confirming: | 80.7692 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGTGTCCCAACCTTGT |
| Suggested primer 2: | CACTGACAGGCTTCCGTCAT |