| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075089 |
| Chromosome: | chromosome 17 |
| Location: | 428158 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698850 | ISA2,STA8 | (1 of 2) 3.2.1.68 - Isoamylase / Debranching enzyme; Isoamylase-type starch debranching enzyme 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCATGGCCTGGGACGGCCGCGCCGGCCCCGGCGGCGAGGGCGCCTGGG |
| Internal bar code: | TTGGTGATACGTGGGACATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 121 |
| LEAP-Seq percent confirming: | 8.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTCCGAGCTGTTTTAC |
| Suggested primer 2: | CAAGGAAGGAGGTACGCAGG |