| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075090 |
| Chromosome: | plastome |
| Location: | 76648 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802295 | ChreCp030,psbN,2717031 | photosystem II protein N; (1 of 1) K02715 - PsbN protein (psbN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTTTACCTTTTTTTTATGGTTTCTTCTGTTAAGTGTAACTGGCTATT |
| Internal bar code: | GGTATGAGCTCACTCTTAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAACCCCTTCCCCTTCGG |
| Suggested primer 2: | GTAGGCAGTCCCTTTTCCCC |