| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075092 |
| Chromosome: | chromosome 12 |
| Location: | 2333011 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508100 | (1 of 2) PTHR21580//PTHR21580:SF21 - SHIPPO-1-RELATED // O(6)-METHYLGUANINE-INDUCED APOPTOSIS 2 | 3'UTR | |
| Cre12.g508150 | SRH21 | (1 of 1) PTHR10799//PTHR10799:SF691 - SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY-RELATED // MYB DOMAIN-CONTAINING PROTEIN-RELATED; SNF2-related DNA/RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAAGCATAAGTTACAGGGTATGCAGGTCAGGTCTCTTGCAGTCGGTA |
| Internal bar code: | GGACAAGTGCGTCCGTAGCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3218 |
| LEAP-Seq percent confirming: | 90.5263 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGTTGAGGAAGAGCAGT |
| Suggested primer 2: | ACCCGTGTCCATCAAGTTCC |