Insertion junction: CLIP2.075105_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.075105
Chromosome:plastome
Location:20904
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802271 ChreCp009,2717052,chlL light-independent protochlorophyllide reductase iron-sulfer ATP-binding protein; (1 of 1) K04037 - light-independent protochlorophyllide reductase subunit L (chlL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCTTATTAAATTTTAAGATAGAAATCTGATAAAAGAGTAAAAAGTTCT
Internal bar code:CGGTCCGCCGGCACTACTTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:462
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGAGGTTATGGGGGTGTA
Suggested primer 2:GGTACCGCCACTGCCTATTT