| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075125 |
| Chromosome: | chromosome 16 |
| Location: | 6938041 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671825 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCGCGCCACGTCTGTCTGGAGGTCCTAGGCACCGGCACCGACGGGGC |
| Internal bar code: | TCTAGTGGAGTCGTTGTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 334 |
| LEAP-Seq percent confirming: | 95.7447 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGAAAGCTATCAGCCAA |
| Suggested primer 2: | CTGCATGAAGGAGCTCACCA |