| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.075143 |
| Chromosome: | chromosome 6 |
| Location: | 2944360 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273413 | (1 of 1) K14835 - ribosomal RNA methyltransferase Nop2 [EC:2.1.1.-] (NOP2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGGAAAGGCGGATTTATCTAAACGATACAAGATTACACTACCAAAT |
| Internal bar code: | TCCGTGCCGGGGACACCTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1647 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGAGTGAGGAAGGTGAA |
| Suggested primer 2: | GCACATCGCAGCACATAGTG |