| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075153 |
| Chromosome: | chromosome 7 |
| Location: | 2974976 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g333100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCACAAGGGATAGCTTACACTGCCTGCGAAATGAGCTCCAGAAGTTCT |
| Internal bar code: | ATCCCTGTCGAACCAAGATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1519 |
| LEAP-Seq percent confirming: | 3.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTAGTGCACGGTACACA |
| Suggested primer 2: | CTCGGCAAGGCTACGAGTAG |