| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.075154 |
| Chromosome: | chromosome 9 |
| Location: | 6556393 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416150 | ASA7 | Mitochondrial F1F0 ATP synthase associated protein 7 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTACTTTCTTACACGATATACATAGTTGTAACCTGAAGTTAAACATGC |
| Internal bar code: | AATCATTTTAGGACAGATGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2449 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGCTTACCTTGACACGTC |
| Suggested primer 2: | GTGATGTGCATTCCGTACGC |