| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.075171 |
| Chromosome: | chromosome 11 |
| Location: | 3771747 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479250 | RANGAP1 | RAN GTPase-activating protein 1; (1 of 1) K14319 - Ran GTPase-activating protein 1 (RANGAP1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTGTGTGCCACCAGCTCCTGCCAGGTTCGAGCGCAATACTTTGCCCT |
| Internal bar code: | AGTGCTGGATCATGTACAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1779 |
| LEAP-Seq percent confirming: | 97.3333 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAGCTTCGGGTTCAAGT |
| Suggested primer 2: | ATTAGCACGTCAAGGGACCG |