| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075181 |
| Chromosome: | chromosome 12 |
| Location: | 194173 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484750 | CLH2 | Chlorophyllase I; (1 of 3) 3.1.1.14 - Chlorophyllase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGCAGGGGGGGGCTGGAGGGCGTGGCAGGAGCACTGCAACGATTGA |
| Internal bar code: | GACAGATTCGATGGATCGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 41.3793 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACAAAGTCCAGCGTCCG |
| Suggested primer 2: | CTTGAGAGCGTGTGGACACT |