| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075181 |
| Chromosome: | chromosome 15 |
| Location: | 2970929 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g643550 | PDX1 | Pyridoxin biosynthesis protein; (1 of 1) K06215 - pyridoxal 5'-phosphate synthase pdxS subunit (pdxS, pdx1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTTTTCCTGCCAAACGCCCACGGTATCGACCCTTTCCCCTTGTGTTC |
| Internal bar code: | GCCGAACCGTTACCGCTGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGACACACACACACACACA |
| Suggested primer 2: | GGTGTGTGTGTGTGTGTGTG |