Insertion junction: CLIP2.075186_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.075186
Chromosome:chromosome 7
Location:4684618
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g345001 (1 of 3) PF01936 - NYN domain (NYN) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGGTACTGGTGAGGGACGGGACCCAACATGCACACCAGGTTGCGTCGC
Internal bar code:TAGCGGGCTCCGGCGACTCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GACCACAGTGACGTTGTTGC
Suggested primer 2:ACTGTTCCTGCAATCGCTCA