| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075192 |
| Chromosome: | plastome |
| Location: | 7118 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802265 | ChreCp003,2716966,chlB | (1 of 1) K04039 - light-independent protochlorophyllide reductase subunit B (chlB); light-independent protochlorophyllide reductase subunit B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGGTAAATAAACTTCCCCTTCGGGGCTGCATGGCAGCAATATTTATA |
| Internal bar code: | GTTCCTAGGACGGAGTCCAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTAACACGCAACACACC |
| Suggested primer 2: | AAATAGGCAGTGGCGGTACC |