| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.075193 |
| Chromosome: | chromosome 3 |
| Location: | 8479308 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212081 | (1 of 3) IPR013328 - 6-phosphogluconate dehydrogenase, domain 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAGCCGTGCATCTAGCCGGCTGAAGAAGGTGGTTTGGTCCGGGACA |
| Internal bar code: | AACGATTAGTGAAGTTTATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1690 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCAAAACCCCCACACAT |
| Suggested primer 2: | GAATTCGTTCCTGGCATCGC |