| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075196 |
| Chromosome: | chromosome 15 |
| Location: | 141794 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g801676 | (1 of 11) 3.6.4.12//3.6.4.13 - DNA helicase // RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGCGGTTTCTCGGGCAAGGCGGAGGCGTCCAGCTTGCCGCCCAAGCT |
| Internal bar code: | TAGGATCGGGTCGTTCGCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4047 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATAAGTTACCGCGCTGC |
| Suggested primer 2: | GTTGTGGTTCGTGCTGTGTC |