| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075197 |
| Chromosome: | chromosome 16 |
| Location: | 985634 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648850 | ADS1 | ADP-sulfurylase; (1 of 1) 2.7.7.53 - ATP adenylyltransferase / Diadenosinetetraphosphate alpha-beta-phosphorylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGTAAATGCTACCCGGCCGAGCTTTGCGCTGCTCCCGGGGAACGCA |
| Internal bar code: | AAATCTATTGGAGATAGATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1842 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGTGTCGGTTTTGCTG |
| Suggested primer 2: | AGTGATGTCCTTTGAGCCCG |