Insertion junction: CLIP2.075198_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.075198
Chromosome:chromosome 6
Location:596596
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g253200 PKY1 Protein tyrosine kinase; (1 of 1) PTHR23257:SF534 - SERINE/THREONINE-PROTEIN KINASE ROCO7-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGTTCATAAGCGCAAGTCCACGCACATCACCGCTTGTGCTCAAGCCA
Internal bar code:TGGATGGAGTTGTACTCTGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3364
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTCGAGAACATGGTGCTG
Suggested primer 2:CTCTAGGCGCTCCTTGATGG