| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075204 |
| Chromosome: | chromosome 10 |
| Location: | 1476828 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428500 | PHX14,P4H2,PFH2 | (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2); Prolyl 4-hydroxylase 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTTGGTCGGACTGTCCCTTCTTGTAATGTCGCATTCATAGATAAGGC |
| Internal bar code: | ATCTCACCCGTATGAATTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1683 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAATGGGGGCAACGGAT |
| Suggested primer 2: | CTGGAGCGGTCATCTAGCAG |