| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075207 |
| Chromosome: | chromosome 12 |
| Location: | 9226188 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542850 | ARD1 | (1 of 1) 1.13.11.54 - Acireductone dioxygenase (Fe(2+)-requiring) / E-2'; Aci-reductone dioxygenase | 3'UTR |
| Cre12.g801489 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACTTGCGCTACTGACTGCCTACTCTCGTGCCGCCTGCAAGCCCACTC |
| Internal bar code: | GTATGTCGGTCGAAGGTATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTCACGGACTCATGG |
| Suggested primer 2: | CGCTTCACCTCAATCGCATG |