| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075219 |
| Chromosome: | chromosome 5 |
| Location: | 272935 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800512 | (1 of 1) IPR011010//IPR013762//IPR023109 - DNA breaking-rejoining enzyme, catalytic core // Integrase-like, catalytic domain // Integrase/recombinase, N-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTAAATTTTGCTTCGCAATGGGCTGCGCTCTCCGGCCTTATTGATCC |
| Internal bar code: | GATTCATGCTGTTCCATTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3947 |
| LEAP-Seq percent confirming: | 87.1429 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGACCTGAGCGTGTATAC |
| Suggested primer 2: | CGTCTCTGTCTCCGATCGTG |