Insertion junction: CLIP2.075226_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.075226
Chromosome:plastome
Location:125911
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802315 psbI,ChreCp051,2717042 photosystem II protein I; (1 of 1) K02710 - photosystem II PsbI protein (psbI) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAAAAATTTTTAATGTTAACATACTTTTTTTAGCAAAAAATTTCTTTG
Internal bar code:TACTTTCAGAGTGCGGTCTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:71
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTAGCTCGTGGTGCTCGTTT
Suggested primer 2:TGCACGGTAGGTATAGGGCA