Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.075244 |
Chromosome: | chromosome 12 |
Location: | 8913659 |
Confidence (%): | 40 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g545800 | ROC55,ROC11 | Rhythm Of Chloroplast 55; (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGACACGGCGGGAGGGACACGGCGGTGAACTTTGGCGCCACTGTTCG |
Internal bar code: | CGCGCTATGCTGTTCTAGAGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 197 |
LEAP-Seq percent confirming: | 29.4118 |
LEAP-Seq n confirming: | 5 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCTGCTTCTTCCTGCTGTA |
Suggested primer 2: | CACGATGGGCTTCTGCAATG |