| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075265 |
| Chromosome: | chromosome 2 |
| Location: | 5182635 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCTTTACATTCACAGGTGCCTGTGCCTGTGCGTGCATTGATGAAGAT |
| Internal bar code: | GGGATTGCGGGAAACTAGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1391 |
| LEAP-Seq percent confirming: | 5.12821 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAGTTTCTCTGACGTGA |
| Suggested primer 2: | AGAACAAAGTTCGCAACCGC |